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Table 4 Protein-protein interactions assayed by Bacterial adenylate cyclase two-hybrid system

From: Trehalose synthesis in Aspergillus niger: characterization of six homologous genes, all with conserved orthologs in related species

Protein

TpsA

TpsB

TpsC

TppA

TpsA

418 (210–863)*

1746 (1582–1799)

113 (77–135)

71 (43–89)

TpsB

1593 (1467–1832)

1776 (1658–1988)

441 (341–560)

581 (322–714)

TpsC

172 (101–244)

688 (315–980)

1214 (861–1551)

80 (67–102)

TppA

429 (167–656)

691 (462–987)

156 (133–198)

83 (58–98)

  1. *Estimated values are in units/mg dry weight bacteria. Values in parentheses are the highest and lowest scores for each based on three to four independent assays. The positive control zip-zip (T18 and T25 fragments of the leucine zipper of GCN4) was scored to 3429 (2938–4270). Negative controls and remaining protein interactions scored at maximum 220 (zip-tpsA) but usually less than 50. Values in bold are considered true protein-protein interactions.