Rapid and sensitive detection of Candidatus Liberibacter asiaticus by loop mediated isothermal amplification combined with a lateral flow dipstick

Table 2 Comparison between Las -LAMP and real time PCR assay sensitivity from DNA purified from a Candidatus Liberibacter asiaticus positive plant

Detection method	Purified DNA from a *Las* positive citrus plant
	100 ng	10 ng	1 ng	100 pg	10 pg	1 pg	100 fg
Las-LAMP Gel	+	+	+	+	+	-	-
Las-LAMP FLD	+	+	+	+	+	-	-
Real time PCR	+	+	+	+	+	-	-

For each dilution the Las-LAMP reaction was performed in triplicate. Gel: gel electrophoresis. LFD: lateral flow dipstick. +: Positive reaction. -: Negative reaction. Real time PCR have been scored as positive if amplification could be detected during the reaction time.

ISSN: 1471-2180