Figure 3

Fast detection of Las from Diaphorina citri. A. Fast psyllid sample preparation procedure: The specimen is grinded by harsh vortexing in 200 μL of the PCR inhibitor chelator matrix InstaGene® (BIORAD™). After that, the sample is incubated at 56°C for 20 minutes to activate the matrix binding groups and subjected to a final incubation at 100°C for 8 minutes to disrupt cell material. A 5-μL aliquot is then used for Las-LAMP. B. Las-LAMP results from an infected psyllid (left) and an uninfected psyllid (right). On Figure 3A, the insect image is representative of a general flying insect for illustration purposes and it is not intended to represent an actual Diaphorina citri psyllid.