Table 2 E. coli strains and plasmids used in this work
Strains and plasmids | Relevant genotype or description | Construction or reference |
|---|---|---|
MC4100 | araD, lac, rpsL, flbB, deoC, ptsF, rbsR, relA1 | [37] |
LSB022 | MC4100 (ppkppx::Km) | [22] |
LSB022/pBC29 | LSB022/pBC29((ppkppx::Km /ppk +, Ap) | [29] |
RKP2935 | RKP4353 [Φ(pitA–lacZ)] pitA::Cm | [38] |
AN3901 | JC7623 pitB::Cm | [39] |
AN4080 | pitA1 pitB::Cm | [39] |
LSB026 | MC4100 pitA:: Cm | (P1(RKP2935)xMC4100) |
MGP001 | MC4100 pitB::Cm | (P1(AN3901)xMC4100) |
JW0473-3 | F-, araD-araB, lacZ, copA::km λ − , rph-1, rhaD-rhaB, hsdR | CGSC |
MGP002 | MC4100 copA::Km | (P1(JW0473-3)xMC4100) |
MGP003 | MGP002 copA::FRT | This study |
MGP004 | MGP003 ppkppx::Km, copA − | (P1(LSB022)xMGP003) |
MGP005 | MGP004 /pBC29 ((ppkppx::Km, copA − /ppk + , Ap) | This study |
pBC29 | (ppk + , Ap) | [24] |
pCP20 | Ap, Cm, cI857 lPR flp pSC101 oriTS | [40] |