Figure 2From: Species-specific viability analysis of Pseudomonas aeruginosa, Burkholderia cepacia and Staphylococcus aureus in mixed culture by flow cytometry Viability analysis by flow cytometry. Viability was determined by membrane integrity analysis using SYBR Green I (dilution of 5 Ă— 103) and PI (5 μg/mL). Representative pseudo color dot plots with defined gates are shown for (A) P. aeruginosa, (B) B. cepacia and (C) S. aureus in stationary growth phase (t = 12 h) from pure culture. Gates were set manually for each species based on SYBR Green I and PI fluorescence signals of isopropanol-treated (red) and untreated cells (green). For each gate region, relative frequencies of total cells are presented. Only events with positive SYBR Green I fluorescence were considered as cells (see Figure 7).Back to article page