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Figure 1 | BMC Microbiology

Figure 1

From: Reliable differentiation of Meyerozyma guilliermondii from Meyerozyma caribbica by internal transcribed spacer restriction fingerprinting

Figure 1

Differentiation of M. guilliermondii and M. caribbica by Taq I digestion of ITS1-5.8S-ITS2. A: Multiple sequence alignment of representative ITS1-5.8S-ITS2 sequences of the two species obtained from NCBI GenBank and CBS yeast database showing position of Taq I recognition site (highlighted) which distinctly differentiated the two species. B: Taq I restriction digestion profile of ITS1-5.8S-ITS2 amplicons obtained from some of the representative isolates. Lane 1: C. guilliermondii ATCC 6260; Lane 2 − 12: isolates of M. guilliermondii genotype group MG (A1S10Y1, A2S10Y1, A3S9Y1, A2S9Y1, A3S11Y1, A3S2Y1, A3S6Y1, A2S6Y1, A1S9Y1, Kw3S3Y1 and Kw2S11Y2); Lane 13 – 20: isolates of M. caribbica genotype group MC (A1S10Y2a, A1S10Y3, A1S10Y5, Kw3S2Y1, Kw2S3Y1, Kw3S3Y3, Kw3S3Y4 and Kw1S7Y2); Lane M: PCR 100 bp Low DNA ladder (Sigma-Aldrich).

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BMC Microbiology

ISSN: 1471-2180

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