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Figure 4 | BMC Microbiology

Figure 4

From: Molecular characterization of KU70 and KU80 homologues and exploitation of a KU70-deficient mutant for improving gene deletion frequency in Rhodosporidium toruloides

Figure 4

Phenotypic and genotypic characterization of pKOCAR2 transformants. (A) A transformation plate showing both red and albino transformants, with black arrow heads marking some albino transformants. (B) Southern blot results using the 5′ flanking sequence of CAR2 as a probe. Genomic DNA was digested with Pvu I and a band shift from 5.0 kb (WT) to 3.0 kb indicates successful deletion of CAR2 gene.

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BMC Microbiology

ISSN: 1471-2180

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