Table 1 Bacterial strains and plasmids used in this study

Strains

E. coli

DH5α

E. coli [F’ Φ80lacZ ∆M15 ∆(lacZYA-argF)U169 deoR recA endA1 hsdR17 (rK-mK+)phoA supE44 lambda- thi-1]

[33]

CECT831

Indicator strain for mangotoxin production

CECT^a

P. syringae pv. syringae

B728a

Complete genome, non-mangotoxin producer

[34]

UMAF0158

Wild type, isolated from mango, mangotoxin producer, Nf^r

[2]

mboA ^-

Derivative mutant of UMAF0158 by insertion in mboA, Km^r, Nf^r (named mbo A^-)

[6]

∆mgoA

Derivative mutant of UMAF0158 by deletion of mgoA, Nf^r (named ∆mgo A)

[7]

2βB7

miniTn5 mutant of UMAF0158 in gacA defective in mangotoxin, Km^r, Nf^r (named gac A^-)

[4]

3αE10

miniTn5 mutant of UMAF0158 in gacS defective in mangotoxin, Km^r, Nf^r(named gac S^-)

[2]

3γH1

miniTn5 mutant of UMAF0158, defective in mangotoxin production, Km^r, Nf^r

[2]

4βA2

miniTn5 mutant of UMAF0158, defective in mangotoxin production, Km^r, Nf^r

[2]

5αC5

miniTn5 mutant of UMAF0158, defective in mangotoxin production, Km^r, Nf^r

[2]

6γF6

miniTn5 mutant of UMAF0158, defective in mangotoxin production, Km^r, Nf^r

[2]

P. protegens Pf-5

Non mangotoxin producer, mbo and mgo operon absent

[35]

Plasmids

pBBR1MCS-5

4.7 kb broad-host-range cloning vector, Gm^r

[36]

pGEM-T

3.0 kb cloning vector, Ap^r

Invitrogen

pGEM-TBCAD

mgo BCAD cloned in pGEM-T, Ap^r

This study

pLac-mgoBCAD

mgo BCAD cloned in pBBR1MCS-5 downstream the lacZ promoter in the vector, mgo operon expression under its own and P _LAC promoter, Gm^r

This study

pLac-mboABCDEF

mboABCDEF cloned in pBBR1MCS-5 downstream the lacZ promoter in the vector, mbo operon expression under its own and P _LAC promoter, Gm^r

[6]

pLac-mboFEDCBA

mboABCDEF cloned in pBBR1MCS-5 in the opposite direction than the lacZ promoter in the vector, mbo operon expression under its own promoter, Gm^r

[6]

pMP220

Promoter-probe vector containing a promoterless LacZ gene, Tet^r

[37]

pMP-mboABCDEF

mboABCDEF cloned in promoter-probe vector containing a promoterless LacZ gene, mbo operon expression under its own promoter, Tet^r

This study

pMP::P _mboI

pMP220 vector containing the mbo operon promoter, Tet^r

[6]