Endophytic bacterial community of grapevine leaves influenced by sampling date and phytoplasma infection process

BMC Microbiology

Table 3 Nucleotide sequences and specificity of primers employed in the present study

Name	Primer sequence (5’-3’)	Gene target	Taxon target	Reference
6S-27F	AGAGTTTGATCCTGGCTCAG	16S rRNA	Bacteria	[19]
338R	GCTGCCTCCCGTAGGAGT	16S rRNA	Bacteria	[19]
α682F	CIAGTGTAGAGGTGAAATT	16S rRNA	α-Proteobacteria	[18]
908αR	CCCCGTCAATTCCTTTGAGTT	16S rRNA	α-Proteobacteria	[18]
1080γF	TCGTCAGCTCGTGTYGTGA	16S rRNA	γ-Proteobacteria	[18]
γ1202R	CGTAAGGGCCATGATG	16S rRNA	γ-Proteobacteria	[18]
Act920F3	TACGGCCGCAAGGCTA	16S rRNA	Actinobacteria	[18]
Act1200R	TCRTCCCCACCTTCCTCCG	16S rRNA	Actinobacteria	[18]
Lgc353	GCAGTAGGGAATCTTCCG	16S rRNA	Firmicutes	[17]
Eub518	ATTACCGCGGCTGCTGG	16S rRNA	Firmicutes	[17]
MB4	CCGCGTGAGTGATGAAGG	16S rRNA	Methylobacterium	[21]
MB	AGCGCCGTCGGGTAAGA	16S rRNA	Methylobacterium	[21]
Sph-spt 694f	GAGATCGTCCGCTTCCGC	spt	Sphingomonas	[22]
Sph-spt 983r	CCGACCGATTTGGAGAAG	spt	Sphingomonas	[22]
Gro1	CTGGAAGACATCGCGATC	groEL	Burkholderia	[20]
Gro2	CGTCGATGATCGTCGTGTT	groEL	Burkholderia	[20]
pagF	CACTGGAAACGGTGGCTAAT	16S rRNA	Pantoea	[23]
pagR	CGGCAGTCTCCTTTGAGTTC	16S rRNA	Pantoea	[23]

ISSN: 1471-2180