Figure 1From: Functional significance of nuclear export and mRNA binding of meiotic regulator Spo5 in fission yeast RNA recognition motifs are essential for the localization and function of Spo5. (A) Localization analyses using truncated mutants of Spo5. Localization of wild-type and mutant proteins of Spo5-GFP (green) during the period between meiosis I and meiosis II was detected using the nuclear envelope marker Cut11-4mRFP (red). Numbers on the right indicate the frequency of cells displaying nuclear GFP signals. Schematic images depict the domains of the mutant proteins. Black boxes depict two RNA-recognition motifs, RRM1 and RRM2, respectively. Scale bar, 5 μm. (B) Sporulation efficiency of specific mutants used in (A), measured at 30°C (n > 500). Error bars indicate standard deviation. (C) Localization analyses using point mutants of Spo5. Localization of Spo5-GFP harboring specific point mutations was observed as in (A). Positions of mutation sites (×) are shown in schematic images. Scale bar, 5 μm. (D) Sporulation efficiency of the strains used in (C), measured at 30°C (n > 500). Error bars indicate standard deviation.Back to article page