Figure 1

Promoter activities of acrA and acrD from Erwinia amylovora. The activity was determined in the course of growth in LB broth, OD₆₀₀, optical density at 600 nm. (A) Relative mRNA transcript abundance of acrA and acrD during cellular growth of Ea1189 as determined by quantitative RT-PCR. The relative mRNA level was related to the highest average value determined for a gene, which was defined as 100%. (B) Expression of acrA and acrD as determined by transcriptional fusions with the reporter gene egfp. E. amylovora wild type was transformed with pBBR.acrA-Pro.egfp and pBBR.acrD-Pro.egfp, respectively. Experiments were performed in triplicates with similar results.