Sequence analysis for detection of drug resistance in Mycobacterium tuberculosis complex isolates from the Central Region of Cameroon

Table 4 Streptomycin and ethambutol resistance-associated mutations detected in M. tuberculosis study isolates

Resistance to	Gene	N° and type of isolates tested	N° of isolates with indicated genotype	Nucleotide change	Amino acid change
Streptomycin	rpsL	27 SM^R	2	43AAG → AGG	Lys → Arg
	rpsL	100 SM^S	0	WT	NA
	gidB	27 SM^R	1	138GCG → CCG	Ala → Pro
	gidB		1	79TTG → TGG	Leu → Trp
			1	75CCG → TCG	Pro → Ser
			1	48CAT → AAT	His → Asn
			1	36GTG → GGG	Val → Gly
		100 SM^S	3	205GCA → GCG	Ala → Ala*
			3	16CTT → CGT	Leu → Arg
Ethambutol	embC	2 EMB^R	0	WT	NA
		100 EMB^S	3	-20A → C	NA
			3	-230A → C	NA
	embA	2 EMB^R	0	WT	NA
		100 EMB^S	3	330CTG → TTG	Leu → Leu*
	embB	2 EMB^R 100 EMB^S	1	306	Met → Val
			0	WT	NA

*: synonymous mutation; NA = not applicable; WT = wild type; SM^R = streptomycin resistant isolate; SM^S = streptomycin sensitive isolate; EMB^R = ethambutol resistant isolate; EMB^S = ethambutol sensitive isolate; N° = Number.

ISSN: 1471-2180