Table 1 Primer sequence used to amplify all four fragments of M. pneumoniae M129 P1 gene
From: Delineation of immunodominant and cytadherence segment(s) of Mycoplasma pneumoniae P1 gene
Primers | Position (bp) | Sequences 5’ to 3’ |
|---|---|---|
F-P1-1 | 1–21 | GGCCATGGGATCCATGCATCAAACCAAAAAAACG |
R-P1-1 | 1051–1069 | CCAAGCTTGTCGACCCAAGGAGTTGGTGATCC |
F-P1-2 | 953–974 | GGCCATGGGATCCATTAAACGGAGTGAAGAGTCA |
R-P1-2 | 1978–1996 | CCAAGCTTGTCGACGTTATTGTGAAAGTAGTA |
F-P1-3 | 1875–1896 | GGCCATGGGATCCTTACGCGAAGACCTGCAGCTC |
R-P1-3 | 3840–3858 | CCAAGCTTGTCGACCGGCTGGGTACTATGGTC |
F-P1-4 | 3729–3749 | GGCCATGGGATCCCTGCACTTGGTGAAACCGAA |
R-P1-4 | 4878–4896 | CCAAGCTTGTCGACTGCGGGTTTTTTGGGAGG |