Target | Primers | Sequence (5’-3’) | Annealing temp (°C) | References |
---|---|---|---|---|
Archaea* | 915f 1386r | GTGCTCCCCCGCCAATTCCT GCGGTGTGTGCAAGGAGC | 59 | [11] |
Methanogen | 86f | GCTCAGTAACACGTGG | 56 | [2] |
1340r | CGGTGTGTGCAAGGAG | |||
The novel RCC species* | 178f | TGGGATCTGGAATGACCCATGG | 56 | This study |
434r | TGAGAAAAGCTAGAACAAATGTCCT | |||
Methanogen※ | 519f | CAGCCGCCGCGGTAA | 57 | [39] |
915r# | CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCG | |||
Methanogen (mcr A gene) | MLf | GGTGGTGTMGGATTCACACARTAYGCWACAGC | 55 | [40] |
 | MLr | TTCATTGCRTAGTTWGGRTAGTT |  |  |