Strains, plasmids or primers | Genotype or description | Reference or source |
---|---|---|
E. coli | Â | Â |
  DH5αλ pir | φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 deoR λpir | [19] |
P. fluorescens | Â | Â |
  Pf0-1 | Wild type, Ampr | [20] |
  PfΔdap | Pf0-1 ΔdapB | This Study |
  Pf0-1::pKNOCK Fr2 | Pf0-1 with partial deletion of Fr2 | This Study |
  Pf0-1::pKNOCK Fr4 | Pf0-1 with partial deletion of Fr4 | This Study |
  Pf0-1::pKNOCK Fr9 | Pf0-1 with partial deletion of Fr9 | This Study |
  Pf0-1::pKNOCK Fr10 | Pf0-1 with partial deletion of Fr10 | This Study |
  Pf0-1::pKNOCK Fr2pJB | Pf0-1 with partial deletion of Fr2 carrying pJB866 | This Study |
  Pf0-1::pKNOCK Fr2pJB Fr2 | Pf0-1 with partial deletion of Fr2 Complemented by pJB866::Fr2 | This Study |
  Pf0-1::pKNOCK Fr10pJB | Pf0-1 with partial deletion of Fr10 carrying pJB866 | This Study |
  Pf0-1::pKNOCK Fr10pJB Fr10 | Pf0-1 with partial deletion of Fr10 Complemented by pJB866::Fr10 | This Study |
Plasmids | Â | Â |
  pGEM-T Easy | Apr; cloning vector for PCR products | Promega |
  pIVETdap | dapB’ cloned in SpeI site of pUIC3 | [11] |
  pJB866 | Broad-host-range vector, Tcr | [21] |
  pRK2013 | Helper plasmid, IncP Tra+ Mob+ ColE1, Kmr | [21] |
  pKNOCK-Km | pBSL63 derivative carrying RP4 oriT and R6K γ-ori, Kmr | [22] |
  pSR47s | Kmr; sacB-containing suicide vector (requiring R6K replication origin) | [23] |