Strain or plasmid | Description and characteristicsa | Reference |
---|---|---|
Strains | Â | Â |
P. aeruginosa | Â | Â |
 PA14 WT | Wild type P. aeruginosa PA14 | [25] |
 PA14 typA | typA insertion mutant of PA14, Gmr | This study |
 PA14 typA::ptypA+ | Complemented mutant PA14 typA harboring plasmid pUCP20::typA+; Gmr, Cbr | This study |
 PA14 pscC | pscC transposon mutant ID29579 of the Harvard PA14 mutant library | [25] |
E. coli | Â | Â |
 DH5α | F–ϕ80lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK– mK+) supE44λ–thi-1 gyrA96 relA | Invitrogen |
Plasmids | Â | Â |
 pUCP20 | E. coli – Pseudomonas shuttle vector for constitutive expression of cloned genes, Cbr | [42] |
 pEX18Ap | Suicide vector for mutant regeneration in Pseudomonas, Ampr/Cbr | [43] |
 pUCP20::typA+ | pUCP20 containing the cloned typA gene; Ampr/Cbr | This study |
 pUCP20::exsA+ | pUCP20 containing the cloned exsA gene; Ampr/Cbr | This study |