Table 1 Strains and plasmids
From: Absence of long-range diffusion of OmpA in E. coliis not caused by its peptidoglycan binding domain
Strains | Genotype | Reference |
|---|---|---|
LMC500 (MC4100 lysA) | F, araD139, Δ (argF-lac)U169, deoC1, flbB5301, ptsF25, rbsR, relA1, rpslL150, lysA1 | [23] |
DH5α | F, endA1, hsdR17(rk mk+), supE44, thi-1, recA1, gyrA, relA1, Δ (lacZYA-argF)U169, deoR, Ф80 lacZΔ M15 | Lab collection |
DH5α-Z1 | DH5α LacIq+ TetR+ | [24] |
Plasmids | Proteins expressed | Reference |
pGI10 | pTHV037 OmpA-LEDPPAEF-mCherry | This work |
pGV30 | pTHV037 OmpA-177-(SA-1)-LEDPPAEF-mCherry | This work |
pSAV47 | pTHV037 mCherry-EFSR | [25] |
pTHV037 | pTRC99A with a weakened IPTG inducible promoter | [26] |