From: Molecular analysis of non-O1/non-O139 Vibrio choleraeisolated from hospitalised patients in China
Gene target | Primer sequence (5’-3’) | Probe | Ta* | Amplicon size (bp) | Reference | |
---|---|---|---|---|---|---|
Forward | Reverse | |||||
ompW | TCCTCAACGCTTCTGTGTGGTAT | ATTGATTTCAACATCCGTGGATT | FAM-TGAAACAACGGCAACCTACAAAGCAGG-BHQ1 | 55 | 92 | This study |
hlyA | AGTGGTCAACCGATGCGATT | TTCAGGATCTGCGCTTTATTGTT | ROX-CCCAAGATTATCGCTTCGTGTTTAACGCA- BHQ2 | 47-55 | 76 | This study |
toxR | GATTCGACAAAGTCCCCACAA | TCGGGCGATCAATTGGTAA | HEX-CGTCAAAACGGTTCCGAAACGCG-BHQ1 | 47-55 | 66 | This study |
ctxAB | CTCAGACGGGATTTGTTAGGCACG | TCTATCTCTGTAGCCCCTATTACG | - | 55 | 303 | [39] |
tcpA (1) # | GTGACTGAAAGTCATCTCTTC | AATCCGACACCTTGTTGGTA | - | 55 | 1248 | [40] |
tcpA (2) # | ATATGCAATTATTAAAACAGC | TTATTATTACCCGTTGTCGG | - | 55 | 1052 | [40] |
ace | AGAGCGCTGCATTTATCCTTATTG | AACTCGGTCTCGGCCTCTCGTATC | - | 55 | 655 | [41] |
zot | GCTATCGATATGCTGTCTCCTCAA | AAAGCCGACCAATACAAAAACCAA | - | 55 | 1000 | [41] |
T3SS (vcsC2) | GGAAAGATCTATGCGTCGACGTTACCGATGCTATGGGT | CATATGGAATTCCCGGGATCCATGCTCT AGAAGTCGGTTGTTTCGGTAA | - | 47-60 | 535 | [16] |
T3SS (vcsV2) | ATGCAGATCTTTTGGCTCACTTGATGGG | ATGCGTCGACGCCACATCATTGCTTGCT | - | 47-55 | 742 | [16] |
NAG-ST | CCTATTCATTAGCATAATG | CCAAAGCAAGCTGGATTGC | - | 47-55 | 215 | [16] |