Figure 5

Histological features of mouse corneas following Sereny tests with SF301 and pic mutants. (A, B) Following inoculation with normal saline, normal corneal epithelium with many layers arranged in an orderly manner can be seen (A: ×50 magnification; B ×400 magnification). (C) After infection with SF301, the corneal epithelium was thinner than that of the control, and vesicular changes (arrowheads) were observed (×100 magnification). (D) Corneal epithelial edema was observed (arrowheads; ×200 magnification). (E) Polymorphic nuclear neutrophilic activity was observed (arrowheads; ×200 magnification). (F) Corneal epithelial derangement and detachment were observed (arrowheads; ×200 magnificaiton). (G) After infection with SF301-∆ pic little damage was observed, but corneal epithelial hyperplasia was noted (arrowheads; ×200 magnification). (H) After infection with SF51, little damage was observed (×200 magnification).