Figure 4

Overall mean fold changes in mRNA expression throughout the time course. A. Mean expression changes in mock-treated and infected DBA/2J and C57BL/6J mice across all 10 target host mRNAs in the 5-day time course of IAV infection. The analysis is based on the same data set as used for Figures 2 and 3. Mean fold change values and 95% confidence intervals (vertical lines) were calculated with the Dunnett’s Modified Tukey-Kramer test, using the dCt values (qRT-PCR) of all 10 host-encoded mRNAs as input. B. Schematic representation of the results shown in panel A. As reflected by the thickness of the lines, overall changes are more pronounced in the DBA/2J strain. Procedure-dependent effects are evident between 6 and 24 h in both strains, but infection-related changes begin to evolve and peak earlier in the DBA/2J strain.