Figure 2

Altered membrane lipid composition of strain PDJ28 following the removal of the glycerol supplement. Strain PDJ28 (ΔgpsA) was labeled with [¹⁴C]acetate in the presence of glycerol to an OD₆₀₀ of 0.6. The cells were then washed and resuspended in media either with (A) or without (B) the glycerol supplement, and after 180 min at 37°C, the cellular lipid composition was determined by 2-dimensional thin-layer chromatography of the extracted lipids. The distribution of radioactivity was determined using a PhosphoImager screen and a Typhoon 9200.