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Table 2 Rates of mutation and unequal sister chromatid recombination in wild-type and mutant haploid strains

From: Alleles of the homologous recombination gene, RAD59, identify multiple responses to disrupted DNA replication in Saccharomyces cerevisiae

Genotype

Mutation rate (10-7)

USCR rate (10-6)

Wild-type

4.0 (3.8, 7.4) [1]

1.0 (0.8, 1.2) [1]

rad51::LEU2

n.d.

1.4 (1.0, 1.8) [+1.4]

rad59::LEU2

7.5 (6.6, 8.6) [+1.9]

0.82 (0.43, 1.4) [-1.3]

rad59-Y92A

4.4 (3.9, 5.3) [+1.1]

1.3 (1.1, 1.8) [+1.3]

rad59-K174A

3.2 (1.8, 5.5) [-1.3]

1.1 (0.85, 2.1) [+1.1]

rad59-F180A

4.8 (4, 6.9) [+1.2]

0.61 (0.47, 0.95) [-1.6]

rad27::LEU2

200 (90, 590) [+50]

47 (39, 100) [+47]

rad27::LEU2 rad59-Y92A

220 (60, 510) [+55]

39 (25, 99) [+39]

rad27::LEU2 rad59-K174A

130 (110, 190) [+32.5]

38 (33, 53) [+38]

rad27::LEU2 rad59-F180A

190 (110, 500) [+47.5]

60 (49, 120) [+60]

  1. Rates of CAN1 mutation or USCR were determined from at least 10 independent cultures as described in the Methods. The 95% confidence intervals are in parentheses. Fold decreases (−) and increases (+) from wild-type are in brackets. n.d. – not determined.
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BMC Microbiology

ISSN: 1471-2180

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