Assessment of glycan interactions of clinical and avian isolates of Campylobacter jejuni

Day, Christopher J; Tram, Greg; Hartley-Tassell, Lauren E; Tiralongo, Joe; Korolik, Victoria

doi:10.1186/1471-2180-13-228

BMC Microbiology

Table 4 Binding of GAG and GAG related structures from the glycan array analysis of twelve C. jejuni strains

From: Assessment of glycan interactions of clinical and avian isolates of Campylobacter jejuni

Glycan ID	Human																		Chicken
	11168			351			375			520			81116			81–176			331			008			019			108			434			506
	RT	37	42	RT	37	42	RT	37	42	RT	37	42	RT	37	42	RT	37	42	RT	37	42	RT	37	42	RT	37	42	RT	37	42	RT	37	42	RT	37	42
12A	+	+	+	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
12B	+	+	+	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
12C	+	+	+	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
12D	+	+	+	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
12E	+	+	+	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
12 F	+	+	+	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
12G	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
12H	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
12I	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
12 J	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
12 K	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
12 L	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
12 M	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
12 N	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
12O	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
12P	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
13A	-	-	-	-	-	-	-	-	-	+	+	+	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
13B	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
13C	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
13D	-	-	-	-	-	-	-	-	-	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
13E	-	-	-	-	-	-	-	-	-	+	+	+	+	+	+	-	-	-	+	+	+	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
13 F	+	+	+	+	+	+	+	+	+	-	-	-	+	+	+	+	+	+	-	-	-	+	+	+	-	-	-	+	+	+	+	+	+	-	-	-
13G	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
13H	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
13I	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
13 J	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
13 K	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
13 L	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
13 M	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
13 N	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
13O	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
13P	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
14A	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
14B	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
14C	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
14D	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
14E	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
14 F	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
14G	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
14H	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+
14I	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+

Each of the strains were analysed at room temperature (left), 37°C (middle) and 42°C (right). Binding +; No binding -. 12A-12 F are Carrageenan repeats; 12G-13E are digested Glycoaminoglycans in their most basic non-repeating unit (12G ΔUA-2S → GlcNS-6S Na4 (I-S); 12H ΔUA → GlucNS-6S Na3 (II-S); 12I ΔUA → 2S-GlcNS Na3 (III-S); 12 J ΔUA → 2S-GlcNAc-6S Na3 (I-A); 12 K ΔUA → GlcNAc-6S Na2 (II-A); 12 L ΔUA → 2S-GlcNAc Na2 (III-A); 12 M ΔUA → GlcNAc Na (IV-A); 12 N ΔUA → GalNAc-4S Na2 (ΔDi-4S); 12O ΔUA → GalNAc-6S Na2 (ΔDi-6S); 12P ΔUA → GalNAc-4S,6S Na3 (ΔDi-disE); 13A ΔUA → 2S-GalNAc-4S Na2 (ΔDi-disB); 13B ΔUA → 2S-GalNAc-6S Na3 (ΔDi-disD); 13C ΔUA → 2S-GalNAc-4S-6S Na4 (ΔDi-tisS); 13D ΔUA → 2S-GalNAc-6S Na2 (ΔDi-UA2S); 13E ΔUA → GlcNAc Na (ΔDi-HA); 13 F-14I are larger repeating Glycoaminoglycans ranging from 4mers to 1.6MDa in size (13 F (GlcAβ1-3GlcNAcβ1-4)n (n = 4); 13G (GlcAβ1-3GlcNAcβ1-4)n (n = 8); 13H. (GlcAβ1-3GlcNAcβ1-4)n (n = 10); 13I (GlcAβ1-3GlcNAcβ1-4)n (n = 12); 13 J (GlcA/IdoAα/β1-4GlcNAcα1-4)n (n = 200); 13 K (GlcA/IdoAβ1-3(±4/6S)GalNAcβ1-4)n (n < 250); 13 L ((±2S)GlcA/IdoAα/b1-3(±4S)GalNAcβ1-4)n (n < 250);13 M (GlcA/IdoAβ1-3(±6S)GalNAcβ1-4)n (n < 250); 13 N (GlcAβ1-3GlcNAcβ1-4)n (n = 4); 130 (GlcAβ1-3GlcNAcβ1-4)n (n = 6); 13P (GlcAβ1-3GlcNAcβ1-4)n (n = 8); 14A (GlcAβ1-3GlcNAcβ1-4)n (n = 10); 14B (GlcAβ1-3GlcNAcβ1-4)n (n = 12); 14C (GlcAβ1-3GlcNAcβ1-4)n (n = 14); 14D(GlcAβ1-3GlcNAcβ1-4)n (n = 16); 14E (GlcAβ1-3GlcNAcβ1-4)n (30000 Da); 14 F (GlcAβ1-3GlcNAcβ1-4)n (107000 Da); 14G (GlcAβ1-3GlcNAcβ1-4)n (190000 Da); 14H (GlcAβ1-3GlcNAcβ1-4)n (220000 Da); 14I (GlcAβ1-3GlcNAcβ1-4)n (1600000 Da); 14 J (GlcA/IdoAα/ IdoASα/β1-4GlcNAc/GlcNS/GlcNAc6Sα1-4)n; 14 K (Glcβ1-4Glc)n; see Additional file 1: Table S1 for full list of glycan names and structures).

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