Figure 3From: Poly-L-glutamate/glutamine synthesis in the cell wall of Mycobacterium bovisis regulated in response to nitrogen availabilityGrowth of the mycobacterial strains in low and high nitrogen broth culture. A. OD600 of wild type M. bovis was inoculated to an initial optical density of 0.006 - 0.008 in 7H9 medium containing (●) low nitrogen (3.8 mM ammonium sulphate) and (▲) high nitrogen (60 mM ammonium sulphate). B. OD600 of wild type M. smegmatis and MSFP in low and high nitrogen broth culture. Wild type M. smegmatis, low nitrogen (■), high nitrogen (□); MSFP, low nitrogen (●), high nitrogen (○). Data is mean ± SD of values obtained from three independent cultures. LN, low nitrogen; HN, high nitrogen.Back to article page