Strains and plasmids | Description | Source or reference |
---|---|---|
Strains | Â | Â |
M. gryphiswaldense MSR-1 | wild-type, Nxr | DSM6361 |
M. gryphiswaldense MSR-1 ΔmamX | mamX deficient mutant, Nxr Gmr | present study |
M. gryphiswaldense MSR-1 CmamX | complementation of ΔmamX, NxrGmrTcr | present study |
E. coli DH5α | endA1 hsdR17 (r- m+) supE44 thi-1 recA1 gyrA (NalR) recA1 Δ (lacZYA-argF)U169 deoR [Ø80ΔdlacZ ΔM15] | [35] |
E. coli S17-1 | thi endA recA hsdR with RP4-2-Tc::Mu-Km::Tn7 integrated in chromosome, Smr | [36] |
Plasmids | Â | Â |
pUCGm | pUC1918 carrying the aacC1 gene, Gmr | [37] |
pSUP202 | suicide vector for M. gryphiswaldense MSR-1, CmrTcr Ampr | [38] |
pSUPpX2 | pSUP202 derivative for mamX deletion, GmrCmrAmpr | present study |
pRK415 | Cloning vector, pRK290 derivative, Tcr | [39] |
pRK415X | pRK415 derivative for mamX expression, Tcr | present study |