Table 1 Selected proteins differentially expressed during X. a. pv. citri biofilm formation
From: Insights into xanthomonas axonopodis pv. citri biofilm through proteomics
Spot no. | Protein name | MOWSE score | Accession no. | Species | Gene ID in Xaca | Predicted MW/pI | Observed MW/pI | Peptide match/ coverage | Fold changeb |
|---|---|---|---|---|---|---|---|---|---|
01 Metabolism | |||||||||
01.02 Nitrogen, sulfur and selenium metabolism | |||||||||
01.02.02 Nitrogen metabolism | |||||||||
60 | NAD(PH) nitroreductase | 111 | Y587_XANC5 | X. c. pv. vesicatoria | XAC0554 | 21.0/5.83 | 20.0/4.6 | 7/31% | −5.6 |
01.05 C-compounds and carbohydrate metabolism | |||||||||
220 | UDP-glucose dehydrogenase | 125 | Q8PGN5_XANAC | X. a. pv. citri | XAC3581 | 43.1/6.18 | 68.0/6.7 | 13/25% | +2.6 |
01.06 Lipid, fatty acid and isoprenoid metabolism | |||||||||
01.06.02 Membrane lipid metabolism | |||||||||
609 | Outer membrane protein (FadL) | 1070 | Q8PRE4_XANAC | X. a. pv. citri | XAC0019 | 47.3/5.18 | 54.0/6.0 | 54/40% | +2.6 |
01.20 Secondary metabolism | |||||||||
533 | Coproporphyinogen-III oxidase, aerobic | 191 | HEM6_XANAC | X. a. pv. citri | XAC4109 | 34.6/5.81 | 48.0/5.4 | 11/30% | −1.5 |
434 | Short chain dehydrogenase | 141 | Q8PME5_XANAC | X. a. pv. citri | XAC1484 | 26.0/5.97 | 29.0/4.5 | 14/34% | −5.1 |
02 Energy | |||||||||
02.04 Glyoxylate cycle | |||||||||
331 | KDPG and KHG aldolase | 163 | Q8PKU5_XANAC | X. a. pv. citri | XAC2067 | 22.9/5.24 | 23.0/4.8 | 7/31% | −2.0 |
02.10 Tricarboxylic-acid pathway | |||||||||
98 | Malate dehydrogenase | 905 | MDH_XANAC | X. a. pv. citri | XAC1006 | 34.9/5.37 | 48.0/4.3 | 46/51% | +1.5 |
121 | Dihydrolipoamide S-succinyltransferase | 136 | Q3BVA5_XANC5 | X. c. pv. vesicatoria | XAC1534 | 42.4/5.87 | 69.0/6.5 | 9/10% | +1.8 |
235 | Citrate synthase | 218 | Q3BPS8_XANC5 | X. c. pv. vesicatoria | XAC3388 | 47.9/5.97 | 68.0/6.6 | 8/20% | +2.6 |
591 | Succinate dehydrogenase flavoprotein subunit | 206 | Q3BTD_XANC5 | X. c. pv. vesicatoria | XAC2077 | 65.8/5.89 | 55.0/4.4 | 18/22% | −7.4 |
02.45 Energy conversion and regeneration | |||||||||
02.45.15 Energy generation | |||||||||
76 | ATP synthase beta chain | 72 | Q2P7Q4_XANOM | X. o. pv. oryzae | XAC3649 | 51.0/5.18 | 32.0/6.1 | 3/8% | −10.7 |
442 | Phosphoglycerate kinase | 688 | AAM38190 | X. a. pv. citri | XAC3347 | 40.9/4.91 | 45.0/6.0 | 47/43% | −1.9 |
422 | NADH-ubiquinone oxidoreductase | 40 | Q3BRN4_XANC5 | X. c. pv. vesicatoria | XAC2699 | 48.8/6.32 | 33.0/4.4 | 8/18% | −3.9 |
11 Transcription | |||||||||
11.04 RNA processing | |||||||||
153 | Polynucleotide phosphorylase | 137 | PNP_XANAC | X. a. pv. citri | XAC2683 | 75.5/5.47 | 28.0/5.9 | 6/3% | −1.5 |
12 Protein synthesis | |||||||||
12.01 Ribosome biogenesis | |||||||||
79 | 50S ribosomal protein L4 | 133 | AAM35856 | X. a. pv. citri | XAC0973 | 21.8/9.68 | 14.0/5.9 | 4/15% | +5.1 |
12.04 Translation | |||||||||
26 | Elongation factor Tu | 294 | Q3BWY6_XANC5 | X. c. pv. vesicatoria | XAC0957 | 43.3/5.45 | 67.0/6.2 | 25/24% | +2.2 |
173 | Elongation factor Tu | 329 | Q3BWY6_XANC5 | X. c. pv. vesicatoria | XAC0957 | 43.3/5.45 | 48.0/5.9 | 20/42% | +4.4 |
14 Protein fate (folding, modification and destination) | |||||||||
14.01 Protein folding and stabilization | |||||||||
416 | Chaperone protein DnaK | 98 | DNAK_XANOM | X. o. pv. oryzae | XAC1522 | 68.9/5.02 | 66.0/6.3 | 10/12% | +2.9 |
20 Cellular transport, transport facilities and transport routes | |||||||||
20.03 Transport facilities | |||||||||
151 | Regulator of pathogenicity factors | 104 | Q8PJM6_XANAC | X. a. pv. citri | XAC2504 | 41.3/5.98 | 41.0/4.3 | 8/21% | +3.2 |
429 | Regulator of pathogenecity factors | 729 | Q8PJM6_XANAC | X. a. pv. citri | XAC2504 | 41.3/5.98 | 47.0/4.5 | 55/61% | +2.7 |
486 | Regulator of pathogenecity factors | 231 | Q8PJM6_XANAC | X. a. pv. citri | XAC2504 | 41.3/5.98 | 48.0/5.2 | 16/30% | +2.2 |
526 | *Regulator of pathogenecity factors | 183 | Q3BS50_XANC5 | X. c. pv. vesicatoria | XAC2504 | 46.4/7.10 | 48.0/5.3 | 16/21% | +1.8 |
555 | *Regulator of pathogenecity factors | 148 | Q3BS50_XANC5 | X. c. pv. vesicatoria | XAC2504 | 46.4/7.10 | 42.0/4.9 | 11/12% | +2.8 |
30 Cellular communication/Signal transduction mechanism | |||||||||
103 | OmpA-related protein | 371 | Q8PER6_XANAC | X. a. pv. citri | XAC4274 | 110.1/5.29 | 75.0/5.9 | 28/16% | +2.9 |
1 | TonB-dependent receptor | 1406 | Q8PI48_XANAC | X. a. pv. citri | XAC3050 | 105.8/4.76 | 42.0/4.1 | 89/34% | +2.9 |
2 | TonB-dependent receptor | 1441 | Q8PI48_XANAC | X. a. pv. citri | XAC3050 | 105.8/4.76 | 58.0/6.7 | 85/35% | +2.9 |
74 | TonB-dependent receptor | 597 | Q8PI48_XANAC | X. a. pv. citri | XAC3050 | 105.8/4.76 | 20.0/4.7 | 27/15% | +3.4 |
219 | TonB-dependent receptor | 356 | Q8PI48_XANAC | X. a. pv. citri | XAC3050 | 105.8/4.76 | 68.0/6.4 | 23/23% | +2.2 |
466 | TonB-dependent receptor-precursor | 113 | Q8PI27_XANAC | X. a. pv. citri | XAC3071 | 97.3/5.14 | 54.0/6.8 | 7/4% | +3.6 |
55 | *TonB-dependent receptor | 166 | Q2HPF0_9XANT | X. a. pv. glycines | XAC3489 | 88.9/4.93 | 58.0/6.4 | 8/9% | +2.8 |
168 | TonB-dependent receptor | 636 | Q8PGX3_XANAC | X. a. pv. citri | XAC3489 | 89.0/5.00 | 55.0/6.0 | 38/29% | +4.9 |
38 | *TonB-dependent receptor | 594 | Q8PHT1_XANAC | X. a. pv. citri | XAC3168 | 87.3/5.20 | 48.0/6.0 | 44/21% | −1.8 |
15 | TonB-dependent receptor | 229 | Q8PH16_XANAC | X. a. pv. citri | XAC3444 | 103.2/4.79 | 66.0/6.4 | 20/14% | −3.5 |
30.01.05.01 Protein kinase | |||||||||
49 | Adenylate kinase | 93 | Q3BPM9_XANC5 | X. c. pv. vesicatoria | XAC3437 | 19.9/5.33 | 18.0/5.9 | 8/24% | −2.4 |
420 | Histidine kinase- 2 component sensor system | 40 | Q3BTZ4_XANC5 | X. c. pv. vesicatoria | XAC1991 | 45.9/5.33 | 48.0/5.5 | 10/13% | −2.2 |
34 Interaction with the environment | |||||||||
86 | YapH protein | 51 | Q8PKM0_XANAC | X. a. pv. citri | XAC2151 | 306.9/4.15 | 68.0/5.5 | 1/0% | +4.1 |
42 Biogenesis of cellular components | |||||||||
42.27 Extracellular/secretion protein | |||||||||
432 | OmpW family outer memb. prot. precursor | 151 | Q3BP00_XANC5 | X. c. pv. vesicatoria | XAC3664 | 23.8/4.97 | 17.0/6.1 | 5/13% | +2.2 |