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Table 4 Kinetic parameters of trifluorothymidine with purified recombinant human TK1, TK2, and Ureaplasma TK*

From: Inhibition of Mycoplasma pneumoniae growth by FDA-approved anticancer and antiviral nucleoside and nucleobase analogs

 

Km(μM)

kcat(s-1)

kcat/Km(s-1M-1)×103

Human TK1

5.9 ± 1.7

0.043 ± 0.003

7.3 ± 1.8

Human TK2

8.8 ± 3.8

0.026 ± 0.003

3.0 ± 0.8

Ureaplasma TK

9.9 ± 5.2

0.055 ± 0.008

5.6 ± 1.5

  1. *Assays were performed using phosphoryl transfer assay with [γ-32P]-labelled ATP (100 μM) and variable concentrations of TFT (1 – 100 μM). The reaction products were separated by thin layer chromatography and were quantified. Thymidine (10 μM) was used as a control. Data are from three independent measurements and are expressed as mean ± SD.
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BMC Microbiology

ISSN: 1471-2180

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