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Table 3 Inhibition of Mpn and human HPRT by purine analogs *

From: Inhibition of Mycoplasma pneumoniae growth by FDA-approved anticancer and antiviral nucleoside and nucleobase analogs

Substrate

Hypoxanthine

Guanine

Analogs

IC50(μM)

IC50(μM)

 

Mpn

Human

P value

Mpn

Human

P value

6-thioguanine

3.5 ± 0.5

20.5 ± 6.5

0.0107

3.16 ± 0.2

39.8 ± 4

<0.0001

6-mercaptopurine

89.7 ± 14.5

22.5 ± 3.6

0.0015

281.8 ± 21

25.1 ± 3

<0.0001

Theophylline

> 4000

1585 ± 134

 

nd

nd

 

Caffeine

> 4000

2511 ± 156

 

nd

nd

 
  1. *Assays were performed with 10 μM tritium labelled hypoxanthine or guanine as substrates and various concentrations of the inhibitors. Data were mean ± standard deviation (SD) from at least three independent determinations. P < 0.05 is considered as significant.
  2. nd = not determined.
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BMC Microbiology

ISSN: 1471-2180

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