Proposal of a quantitative PCR-based protocol for an optimal Pseudomonas aeruginosa detection in patients with cystic fibrosis

Table 2 Primers and probes used in this study for the detection and quantification of Pseudomonas aeruginosa

Name	Sequence (5′- 3′)*	DNA target	Reference
OPRL-F	AACAGCGGTGCCGTTGAC	oprL	[30]
OPRL-R	GTCGGAGCTGTCGTACTCGAA	oprL	[30]
oprL-MGB	fam -TGAGCGACGAAGCC-bhq	oprL	[30]
gyrB-F	CCTGACCATCCGTCGCCACAAC	gyrB	[19, 36]
gyrB-F	CGCAGCAGGATGCCGACGCC	gyrB	[19, 36]
gyrB-TM	fam-CCGTGGTGGTAGACCTGTTCCCAGACC-bhq	gyrB	[14]
ecfX-F	CGCATGCCTATCAGGCGTT	ecfX	[14]
ecfX-R	GAACTGCCCAGGTGCTTGC	ecfX	[14]
ecfX-TM	yak-ATGGCGAGTTGCTGCGCTTCCT-bhq	ecfX	[14]

ISSN: 1471-2180