Figure 1

Construction of mutant strains. ORFs are indicated by boxed arrows (not drawn to scale). The locations of the primers used to amplify the fragments and generate the deletions are indicated by solid arrows. The dash line box indicated the location of the deletion of chromosomal sequence and insertion of an antibiotic resistant cassette (cat or aphA3). (a), (b), (c), and (d) are diagrams for operons cj0309c-cj0310c, cj0423-cj0425, cj1169c-cj1170c and cj1173-cj1174, respectively.