Table 3 Strains and plasmids used in this study
E. colistrain or plasmid | Descriptiona | Reference or sourceb |
|---|---|---|
Strains | ||
W | Wild-type E. coli W | ATCC 9637 |
W Δgsp::Kan | W ΔgspC-M::FRT-kan-FRT | This work |
W Δgsp::FRT | W ΔgspC-M::FRT, derived by FLP recombination from W Δgsp::Kan | This work |
W ΔpppA::Kan | W ΔpppA::FRT-kan-FRT | This work |
W ΔpppA::FRT | W ΔpppA::FRT, derived by FLP recombination from W ΔpppA::Kan | This work |
W ΔsslE::Kan | W ΔsslE::FRT-kan-FRT | This work |
W ΔsslE::FRT | W ΔsslE::FRT, derived by FLP recombination from W ΔsslE::Kan | This work |
Plasmids | This work | |
pRH21 | pACYC184-derived; trc promoter; lacIq | This work |
pRH31 | pTrc99A-derived; trc promoter; lacIq | This work |
pMSD6 | pRH21 with sslE cloned into the MCS | This work |
pMSD7 | pRH21 with sslE lacking the signal peptide-encoding sequence cloned into the MCS | This work |
pMSD8 | pRH21 with pppA cloned into the MCS | This work |
pRH153 | pRH31 with an sslE-cel45A fusion cloned into the MCS | This work |
pRH154 | pRH31 with an sslE-pel10A fusion cloned into the MCS | This work |