Enzymatic and molecular characterisation of leucine aminopeptidase of Burkholderia pseudomallei

BMC Microbiology

Table 1 Effect of metallic ions and inhibitors on LAP activity of B. pseudomallei NCTC 13178

Compound	Concentration	Relative activity (%)
Control		100
Mn²⁺	10 mM	52.2
Zn²⁺	10 mM	42.8
Ca²⁺	10 mM	126.0
Mg²⁺	10 mM	135.8
K⁺	10 mM	107.2
Na⁺	10 mM	118.0
EDTA	2 mM	0
	10 mM	0
1,10-phenanthroline	2 mM	0
	10 mM	0
Phenylmethylsulfonylfluoride (PMSF)	2 mM	69.9
	10 mM	35.9
Amastatin	2 mM	0

ISSN: 1471-2180