Detection and characterization of Wolbachia infections in laboratory and natural populations of different species of tsetse flies (genus Glossina)

Table 3 Wolbachia WSP HVR profiles for 11 populations of Glossina

Code	Species	Country (area, collection date)	wsp	HVR1	HVR2	HVR3	HVR4
12.3A	G. m. morsitans	Zambia (MFWE, Eastern Zambia, 2007)	548	192	9	12	202
32.3D	G. m. morsitans	Zimbabwe (Makuti, 2006)	356	142	9	12	9
GmcY	G. m. centralis	Yale lab-colony (2008)	550	193	9	221	202
30.9D	G. m. morsitans	Zimbabwe (Rukomeshi, 2006)	356	142	9	12	9
GmmY	G. m. morsitans	Yale lab-colony (2008)	548	192	9	12	202
24.4A	G. m. morsitans	KARI-TRC lab-colony (2008)	549	142	9	223	9
09.7G	G. brevipalpis	Seibersdorf lab-colony (1995)	11	9	9	12	9
05.2B	G. austeni	South Africa (Zululand, 1999)	551	180	40	210	18
GauK	G. austeni	Kenya (Shimba Hills, 2010)	507	180	40	210	18
15.5B	G. pallidipes	Ethiopia (Arba Minch, 2007)	552	195	224	224	63
405.11F	G. p. gambiensis	Guinea (Kindoya, 2009)	553	194	223	222	220

WSP profiles of Wolbachia for 11 populations of Glossina, defined as the combination of the four HVR amino acid haplotypes. Each WSP amino acid sequence (corresponding to residues 52 to 222 of the wMel sequences) was partitioned into four consecutive sections, whose breakpoints fall within conserved regions between the hypervariable regions, as follows: HVR1 (amino acids 52 to 84), HVR2 (amino acids 85 to 134), HVR3 (amino acids 135 to 185), and HVR4 (amino acids 186 to 222) [41].

ISSN: 1471-2180