Figure 7From: Mutagenesis of RpoE-like sigma factor genes in Bdellovibrio reveals differential control of groEL and two groES genesTranscriptional expression patterns of the three Bdellovibrio chaperonin genes during axenic Host-Independent growth. RT-PCR with transcript specific primers was carried out on matched concentrations of RNA (matched by Nanodrop spectrophotometer readings) from axenically grown Host-Independent Bdellovibrio. Three independently isolated strains of each sigma factor mutant and each host-independent (HI) wild-type were used to account for HI strain-to strain variation. L- NEB 100 bp ladder –ve - no template negative control + ve- HD100 genomic DNA positive control.Back to article page