Table 1 Primers for Quantitative PCR

L. species

Zariffard MR [28]

F-LBF: 5′- ATGGAAGAACACCAGTGGCG-3′

16 S r RNA

15 min 95 °C, (15 sec 95 °C, 45 sec 50 °C, 45 sec 72 °C) x37

150 nM

R- LBR: 5′- CAGCACTGAGAGGCGGAAAC-3′

L. crispatus

Byun R [29]

LcrisF: 5′-AGCGAGCGGAACTAACAGATTTAC-3′

16 S r RNA

15 min, 95 °C, (15 sec 95 °C, 60 sec 60 °C, 20 sec 72 °C) x40

100 nM

LcrisR : 5′-AGCTGATCATGCGATCTGCTT-3′

L. gasseri

Tamrakar R [30]

LgassF: 5′-AGCGAGCTTGCCTAGATGAATTTG-3′

16 S r RNA

15 min 95 °C, (15 sec 95 °C, 60 sec 57 °C, 60 sec 65 °C) x40

200 nM

LgassR: 5′-TCTTTTAAACTCTAGACATGCGTC-3′

L. iners

De Backer E [31]

InersFw: 5′-GTCTGCCTTGAAGATCGG-3′

16 S r RNA

15 min 95 °C, (15 sec 95 °C, 55 sec 60 °C, 60 sec 65 °C) x35

200 nM

InersRev: 5′-ACAGTTGATAGGCATCATC-3′

L. jensenii

Tamrakar R [30]

LjensF: 5′-AAGTCGAGCGAGCTTGCCTATAGA-3′

16 S r RNA

15 min 95 °C, (15 sec 95 °C, 55 sec 60 °C, 60 sec 72 °C) x40

300 nM

LjensR: 5′-CTTCTTTCATGCGAAAGTAGC-3′

L. vaginalis

In-house designed primers

LV16s_23s_F: 5′-GCCTAACCATTTGGAGGG-3′

16 S-23 S r RNA

15 min 95 °C, (15 sec 95 °C, 30 sec 56 °C, 30 sec 72 °C)x37

200 nM

LV16s_23s_R3: 5′-CGATGTGTAGGTTTCCG-3′

G. vaginalis

Zariffard MR [28]

F-GV1: 5′-TTACTGGTGTATCACTGTAAGG-3′

16 S r RNA

15 min 95 °C, (45 sec 95 °C, 45 sec 55 °C, 45 sec 72 °C) x50

260 nM

R-GV3: 5′-CCGTCACAGGCTGAACAGT-3′

A. vaginae

De Backer E [31]

ATOVAGRT3Fw: 5′-GGTGAAGCAGTGGAAACACT-3′

ATOVAGRT3Rev: 5′-ATTCGCTTCTGCTCGCGCA-3′

16 S r RNA

15 min 95 °C, (20 sec 95 °C, 45 sec 60 °C, 45 sec 72 °C) x45

300 nM