Figure 3

Clustering result with HOPACH using the average linkage distance between clusters is shown. The eight pathogen-exposed samples are clustered according to the dendrogram on the left and cluster into five groups, 1) Y. pestis (KIM5, NYC, and India), 2) Y. pseudotuberculosis, 3) Y. enterocolitica, 4) B. anthracis (Ames and Sterne), and 5) Control. Sixteen cytokines (Eotaxin, IL-10, IL-12(p40), IL-15, IL-1α, IL-1β, IL-6, IL-8, IP-10, MCP-1, MIG, TNFα, TRAIL, sCD23, sCD95, and sICAM-1) are also reordered based on their correlations according to the dendrogram on the top. Clusters go from root at top to leaf node for each cytokine. Clusters in between are based on their agglomerative . The branch shows the similarity, the short the branch, the more similar. In addition, the eight rightmost proteins form a cluster that may involve inflammation-related cascades initiated by an innate immune response to these pathogen. Colors represent units of log10 [pg/ml], in ten equally spaced intervals increasing from white to dark red. A key showing the specific log10 values for each interval is shown in the figure.