Figure 1
From: Coverage evaluation of universal bacterial primers using the metagenomic datasets
Influence of a single mismatch occurring in the last 4 nucleotides. The black column denotes the non-coverage rate when no mismatches were allowed in the last 4 nucleotides, while the white column denotes the rate when a single mismatch was allowed. A Domain non-coverage rates for 8 primers in the RDP dataset; B Phylum non-coverage rates for primer 338 F in the RDP dataset; C Phylum non-coverage rates for primer 519 F in the RDP dataset. Refer to Additional file 1: Figure S1A for the normalized results of Figure 1A.