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Table 1 B. pseudomallei P2-like prophage distribution screen

From: φX216, a P2-like bacteriophage with broad Burkholderia pseudomallei and B. malleistrain infectivity

  

P2-like prophage PCR probe results

  

Multiplex

   

B. pseudomallei

Candidate P2-like prophage group a

P2-like 1

P2-like 2

P2-like group A

φX216 scrnA

φX216 scrnB

GI2

Strains with high φX216 plaquing efficiencyb66 .7% (22/33) P2-like prophage candidate positive strains

 

2668a

φ52237-like

+

+

+

+

+

+

E0237 c

φ52237-like

+

+

+

+

+

+

E0394

φ52237-like

+

+

+

+

+

+

1026b d

φ52237-like

+

+

+

+

+

+

708a

φ52237-like e

+

+

+

+

-

+

2618a

P2L-A

+

+

+

-

-

+

2661a

P2L-A

+

+

+

-

-

+

2692a

P2L-A

+

+

+

-

-

+

2717a

P2L-A

+

+

+

-

-

+

E0021

P2L-A

+

+

+

-

-

+

E0235

P2L-A

+

+

+

-

-

+

E0279

P2L-A

+

+

+

-

-

+

E0345

P2L-A

+

+

+

-

-

+

E0384

P2L-A

+

+

+

-

-

+

E0386

P2L-A

+

+

+

-

-

+

K96243 f

P2L-A

+

+

+

-

-

+

S13 g

P2L-A

+

+

+

-

-

+

2698a

P2L

+

+

-

-

-

-

2704a

P2L h

+

+

-

-

+

-

E0342

P2L

+

+

-

-

-

-

E0366

P2L

+

+

-

-

-

-

E0377

P2L

+

+

-

-

-

-

2613a

 

-

-

-

-

-

ND i

2667a

 

-

-

-

-

-

ND

2673a

 

-

-

-

-

-

ND

2682a

 

-

-

-

-

-

ND

2769a

 

-

-

-

-

-

ND

E0016

 

-

-

-

-

-

ND

E0034

 

-

-

-

-

-

ND

E0241

 

-

-

-

-

-

ND

E0356

 

-

-

-

-

-

ND

E0411

 

-

-

-

-

-

ND

MSHR305

 

-

-

-

-

-

ND

Strains with low φX216 plaquing efficiency j17. 4% (4/23), P2-like prophage candidate positive strains

 

2625a

φ52237-like

+

+

+

+

+

+

2670a

P2L-A

+

+

+

-

-

+

E0037

P2L-A

+

+

+

-

-

+

E0380

P2L-A

+

+

+

-

-

+

2637a

 

-

-

-

-

-

ND

2650a

 

-

-

-

-

-

ND

2660a

 

-

-

-

-

-

ND

2685a

 

-

-

-

-

-

ND

2708a

 

-

-

-

-

-

ND

2719a

 

-

-

-

-

-

ND

2764b

 

-

-

-

-

-

ND

E0024

 

-

-

-

-

-

ND

E0031

 

-

-

-

-

-

ND

E0181

 

-

-

-

-

-

ND

E0371

 

-

-

-

-

-

ND

E0372

 

-

-

-

-

-

ND

E0378

 

-

-

-

-

-

ND

E0383

 

-

-

-

-

-

ND

E0393

 

-

-

-

-

-

ND

1710a

 

-

-

-

-

-

ND

1710b k

 

-

-

-

-

-

-

1106b

 

-

-

-

-

-

ND

406e

 

-

-

-

-

-

ND

Non-φX216 plaquing strains 25. 0% (4/16), P2-like prophage candidate positive strains

 

2671a

P2L-A

+

+

+

-

-

+

2674a

P2L-A

+

+

+

-

-

+

2677a

P2L-A

+

+

+

-

-

+

Pasteur 6068

P2L-A

+

+

+

-

-

+

2614a

 

-

-

-

-

-

ND

2617a

 

-

-

-

-

-

ND

2640a

 

-

-

-

-

-

ND

2665a

 

-

-

-

-

-

ND

2689b

 

-

-

-

-

-

ND

2694a

 

-

-

-

-

-

ND

E0008

 

-

-

-

-

-

ND

E0183

 

-

-

-

-

-

ND

E0350

 

-

-

-

-

-

ND

E0396

 

-

-

-

-

-

ND

1106a

 

-

-

-

-

-

ND

MSHR668

 

-

-

-

-

-

ND

  1. a φ52237-like assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2, P2-like group A, and individual PCR probes φX216 scrnA, φX216 scrnB and GI2. P2L-A assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2, P2-like group A and individual PCR probe GI2. P2L assignment; positive PCR amplicons from multiplex probes P2-like 1, P2-like 2.
  2. bConfluent lysis when spot tested with ~106 pfu φX216.
  3. cφX216 source strain.
  4. d1026b φ52237-like prophage is split into two segments and likely non-functional [15].
  5. eP2-like prophage group cannot be determined based on PCR results. May be P2L-A or φ52237-like.
  6. fφK96243 prophage (group P2-A) located at GI2 [9].
  7. gEncodes the predicted prophage PI-S13-1 (group P2-A) [88].
  8. hP2-like prophage group cannot be assigned based on PCR results. May be P2L or φ52237-like.
  9. IND, GI2 probe results not determined.
  10. jNon-confluent lysis / individual plaques when spot tested with ~ 106 pfu φX216.
  11. kThe strain 1710b genome does not contain a P2-like prophage or prophage insertion at GI2.
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BMC Microbiology

ISSN: 1471-2180

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