Strain or plasmid | Description | Reference or source |
---|---|---|
RN6390B | Standard laboratory strain | NARSAa |
RN4220 | 8325-4 r- | NARSA |
ΔluxS | RN6390B luxS::ermB | This study |
RN6911 | RN6390B derivative; agr locus replaced with tetM cassette | NARSA |
ΔagrΔluxS | RN6911 luxS::ermB, agr/luxS double mutant | This study |
ΔluxSpluxS | Complemented strain of ΔluxS; Apr Cmr | This study |
RN6390BG | RN6390B/pgfp | This study |
ΔluxSG | ΔluxS/pgfp | This study |
RN6911G | RN6911/pgfp | This study |
ΔagrΔluxSG | ΔagrΔluxS/pgfp | This study |
NCTC8325 | Standard Laboratory strain | NARSA |
NCTC8325ΔluxS | NCTC8325 luxS::ermB | 60 |
E. coli strains | Â | Â |
TOP10 | Cloning | Invitrogen |
Plasmids | Â | Â |
pEASY-Blunt | Clone vector, Kanr Apr | Transgen |
pBTluxS | Vector used for luxS mutagenesis, Apr Cmr Emr | 60 |
pLI50 | E. coli-S. aureus shuttle cloning vector, Apr Cmr | Addgene |
pLIluxS | pLI50 with luxS and its promoter, Apr Cmr | 60 |
pgfp | gfp expression with the promoter of S10 ribosomal gene, Apr, Cmr | Â |