Table 2 Hydrogenase activity induced by R. leguminosarum strains in microaerobic cultures and in pea bacteroids
From: Dual role of HupF in the biosynthesis of [NiFe] hydrogenase in Rhizobium leguminosarum
Strain | Genotype | Hydrogenase activitya | ||
|---|---|---|---|---|
1% O2 | 3% O2 | Bacteroids | ||
UPM1155(pALPF1) | wild type | 13440 ± 1720 | 4970 ± 472 | 5980 ± 852 |
UPM1155 (pALPF14) | ΔhypC | < 200 | < 200 | < 200 |
UPM1155 (pALPF5) | ΔhupF | < 200 | < 200 | 470 ± 84 |
UPM1155 (pALPF5/pPM501) | ΔhupF/hupFST | 12890 ± 230 | 5900 ± 779 | 5350 ± 728 |
UPM1155(pALPF5/pPM501C) | ΔhupF/hupFCST | 4780 ± 228 | 1025 ± 255 | 5400 ± 683 |