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Table 5 Interpretation of FungiQuant results for detecting fungal DNA (i.e., rejecting the Null Hypothesis) based on triplicate 5 μl and 10 μl reactions

From: FungiQuant: A broad-coverage fungal quantitative real-time PCR assay

Triplicate pattern

Probability under the Null Hypothesis (Ho: human DNA only)

10 μl Reaction

 

+++

<0.001

++−

0.008

+−−

0.077

---

0.744

5 μl Reaction

 

+++

0.006

++−

0.026

+−−

0.120

---

0.557