Biogenic amine production by the wine Lactobacillus brevis IOEB 9809 in systems that partially mimic the gastrointestinal tract stress

Table 2 Production of biogenic amines in presence of epithelial cells

Precursors added	Bacteria +Human cells		Bacteria		Human cells
	Put (μM)	Tym (μM)	Put (μM)	Tym	Put (μM)	Tym(μM)
Agm (4.3 mM)	1980±170^a	ND	190±80^c	ND	10±2^d	ND
Tyr (10 mM)	ND	180±9^a	ND	220±1^ab	ND	ND
Tyr (10 mM) + Agm (4.3 mM)	1330±420^a	230±9^ab	1003±41^b	320±80^b	7±0^d	ND

Tyramine (Tym) and putrescine (Put) were detected by RP-HPLC in samples containing DMEM medium supplemented or not with 10 mM tyrosine, 4.38 mM agmatine or both precursors, after 8 h incubation. Cells present during the assay: Bacteria + Human cells: L. brevis IOEB 9809 (10⁸ CFU mL^-1) and Caco-2 cells (10⁵ cells mL^-1); Bacteria: L. brevis IOEB 9809 (10⁸ CFU mL^-1) and Human cells: Caco-2 cells (10⁵ cells mL^-1). Results are expressed as the mean ± standard deviation of three independent experiments. ND: not detected. Detection limits: for Put > 2 nM and for Tym > 2.5 nM. Putrescine and tyramine were below the detection limits in the DMEM medium as well as in samples containing either bacteria or Caco-2 cells in absence of the corresponding BA precursor. Differences were assessed by Anova test. Different superscript letters associated with values of the same BA indicate statistically significant differences (P < 0.05).

ISSN: 1471-2180