Genotype or relevant features | Source or reference | |
---|---|---|
Strains: | ||
E. coli: | ||
DH-5α | F- φ80d lacZΔM15Δ (lacZY-argF) U169 deoR recA1 endA1 hsdR17(rk- mk+) phoA supE44l- thi-1 gyrA96 relA1 | [52] |
X. dendrorhous: | ||
UCD 67-385 | ATCC 24230, wild type. | ATCC |
Diploid strain [30] | ||
385-cyp61(+/−) | (385-CYP61/cyp61hph). Heterozygote transformant derived from UCD 67–385 containing an allele of the CYP61 locus interrupted with a hygromycin B resistance cassette. | This work |
385-cyp61(−/−) | (385-cyp61hph/cyp61zeo). Homozygote transformant derived by transformation of 385-cyp61+/− with both CYP61 alleles interrupted, one with a hygromycin B resistance cassette and the other with a zeocin resistance cassette. | This work |
CBS 6938 | ATCC 96594, wild type. | ATCC |
CBS-cyp61(−) | (CBS-cyp61hph). Hemizygote transformant derived from CBS 6938. The single CYP61 locus was interrupted with a hygromycin B resistance cassette. | This work |
AVHN2* | Chilean native isolate, wild type. | Our Lab collection |
Av2-cyp61(−) | (Av2-cyp61zeo). Hemizygote transformant derived from AVHN2. The single CYP61 locus was interrupted with a zeocin resistance cassette. | This work |
UCD 67-210 | ATCC 24202, wild type (Phaffia rhodozyma) | ATCC |
VKM Y-2786 | Wild-type strain. | VKM |
ANCH03* | Chilean Antarctic native isolate, wild type. | Our Lab collection |
ANCH07* | Chilean Antarctic native isolate, wild type. | Our Lab collection |
ANCH10* | Chilean Antarctic native isolate, wild type. | Our Lab collection |
Plasmids: | ||
pBluescript SK- (pBS) | ColE1 ori; AmpR; cloning vector with blue-white selection | Stratagene |
pMN-hph | pBS containing at the EcoRV site a cassette of 1.8 kb bearing the E. coli-Hygromycin B resistance (hph) gene under EF-1 α promoter and GPD transcription terminator of X. dendrorhous. | [31] |
pIR-zeo | pBS containing at the EcoRV site a cassette of 1.2 kb bearing the Streptoalloteichus hindustanus Zeocin resistance Sh ble gene under EF-1 α promoter and GPD transcription terminator of X. dendrorhous. | This work |
pBS-gCyp61 | pBS containing at the EcoRV site a 4,224 bp DNA fragment containing the X. dendrorhous CYP61 gene amplified by PCR with primers CYP61up2.F and CYP61dw2.R. | This work |
pBS-cyp61/Hyg | pBS-gCyp61 bearing the Hygromycin B resistance cassette at the EcoRV site that interrupts the CYP61 gene. | This work |
pBS-cyp61/Zeo | pBS-gCyp61 bearing the Zeocin resistance cassette at the EcoRV site that interrupts the CYP61 gene. | This work |
pBS-cCyp61 | pBS bearing the cDNA of the CYP61 gene. The cDNA measures 1,752 bp with an ORF of 1,581 bp. | This work |