Application | Name | Oligonucleotide sequence (5’ to 3’) |
---|---|---|
Strain construction | PKD13sspAUS2 | ACTATCATCCAATTTTCTGCCCAAATGTCGGGTATTGCTCAGGAGGTTTCTTTCATGATGTCCGGGGATCCGTCGACCTGC |
 | PKD13sspADS | AGATTAACTCCGGCCCAGACGCATTTCACGTTCTGCTTCAGTGTAGGCTGGAGCTGCTTCGA |
 | sspABUS | GATTGGATCCGGCTTCGTTACTCGTGACGCT |
 | sspABDS | GATTGAAGCTTACTTCACAACGCGTAATGC |
 | hnsΔ92-1 | GAGCTGCTGAATAGCCTTGCCGCCGTTAAATCTGGCACCAAAGCTAAACGTGCTTGTGTAGGCTGGAGCTGCTTCG |
 | hnsΔ92-2 | GGTTTTAGTTTCGCCGTTTTCGTCAACGTAGCTATATTTTGCCGGACGCTTATGAATATCCTCCTTAGTTC |
 | hnsUS2 | CCATGGAATTCACCATGAGCGAAGCACTTAAAATTCTGAACA |
 | hnsDS2 | CCAGGTCTGCAGTTATTGCTTGATCAGGAAATCG |
Plasmid construction | lerUS2 | GATTGGATCCGCTGCGACTGCGTTCGCTTGCT |
 | lerDS2 | GATTGAAGCTTCCAGCTCAGTTATCGTTAT |
Primer extension | lerPE | GCTTCCTGCTGTAGA |
 | LEE2PE | GCTGCTTCCATTGATCTTTCTCC |
 | LEE3PE | CAATTTCAACACGGTTATC |
 | LEE4PE | CAGATGCGGGGTTTTGATTAAATTC |
 | LEE5PE | CATTGGGATTATGACCAAGA |
 | mapPE | GGACTAAACATGCTATAAACC |
 | grlRPE | CCTTCCCCACAGGAGTCTTC |
 | stcEPE | GGCAAGGATCGTACATGA |
 | ompAPE | ACGAGATAACACGGTTAAATCC |