Table 3 Oligos used in this work
From: Tigecycline challenge triggers sRNA production in Salmonella enterica serovar Typhimurium
Name | Sequence 5’-3’ | Ref |
|---|---|---|
For deletion of sY20 in SL1344 | ||
sYJ20_Cm_F | CTTGATTGCTGCCCGGCAACAAAA TCACTACACTAACGCCGTGTAGGC TGGAGCTGCTTC | This work |
sYJ20_Cm_R | CTTTGCACCTCAGTTAAAGAGTGG CAAAGGACTTGAGATGGGAATTAG CCATGGTCC | Â |
For cloning sYJ20 coding sequence onto pACYC177 | ||
sYJ20_HF | CCCAAGCTTCTTGATTGCTGCCCGG CAACAA | This work |
sYJ20_BR | CGGGATCCCTTGAGAGGTAGCCTC AAATCCCTT | Â |
For the northern blot assays | ||
NsYJ5 | AGGTTTTACTGCTCGTTTTTCA | This work |
NsYJ20 | ATCCGGATCAGGTTCGACGGGTAT | Â |
NsYJ75 | GCGGGGATTTCTTCCCTTGC | Â |
NsYJ118 | TGTGTTTCAATTTTCAGCTTGATCCAGATT | Â |
5S | CTACGGCGTTTCACTTCTGAGTTC | [53] |
For qPCR | ||
16SF | GTTACCCGCAGAAGAAGCAC | This work |
16SR | CTACGCATTTCACCGCTACA | Â |
tbpAF | GGCTGGAAAAACGACACATT | Â |
tbpAR | TAGACTTTGCGCATCCACAG | Â |
ycfR-F | TGCCGTACTGAGTTCGCTCT | Â |
ycfR-R | GGGCCGGTAACAGAGGTAAT | Â |
dinF-F | TTACTGGGGCTGGTCGATAC | Â |
dinF-R | GCCAGCAATAACGGTTGAAT | Â |
q5S-F | CATGCCGAACTCAGAAGTGA | Â |
q5S-R | AGTTCCCTACTCTCGCATGG | Â |
For 5’RACE | ||
GSP1 | GGCGAAATAGCCGTAAT | This work |
GSP2 | GGGCACCTTGACCGCTTCAT | Â |
GSP3 | GCCACGCCGCTTTTGGCAAA | Â |