Table 1 Bacterial strains, mammalian cells and plasmids used in this study

E.coli strains

DH5α

SupE44ΔlacU169 (Φ80lacZΔM15) hsdR17recA1 endA1 gyrA96 thi-1 relA1

[48]

SM10λpir

Expression of the π protein for replication of suicide vector

[49]

Bordetella strains

RB50

Rabbit

Complex-I strain, ST-12,Sm^r

[20]

RB50ΔbscN

WD3

RB50ΔbscN, Sm^r

[15]

RB50ΔbteA

RB50ΔbteA, Sm^r

[11]

A309

SBL-F6116

Human

Complex-IV strain, ST-9, Sm^r

[10]

A310

SBL-F6368

Human

Complex-IV strain, ST-8, Sm^r

[10]

A345

GA96-01

Human

Complex-IV strain, ST-21, Sm^r

[10]

Bbr69

591

Dog

Complex-IV strain, ST-22, Sm^r

[10]

Bbr77

675

Human

Complex-IV strain, ST-18, Sm^r

[10]

D444

MO149

Human

Complex-IV strain, ST-15, Sm^r

[10]

D445

MO211

Human

Human complex-IV strain, ST-17, Sm^r

[10]

D446

MO275

Human

Complex-IV strain, ST-3, Sm^r

[10]

D758

00-P-2730

Human

Complex-IV strain, ST-34, Sm^r

[10]

D445ΔbscN

Complex-IV strain, ST-17, Sm^r

This study

D445ΔbteA

Complex-IV strain, ST-17, Sm^r

This study

Bbr77ΔbscN

Complex-IV strain, ST-18, Sm^r

This study

Bbr77ΔbteA

Complex-IV strain, ST-18, Sm^r

This study

Bbr68

590

Dog

Complex-I strain, ST-10, Sm^r

[10]

Bbr78

680

Koala bear

Complex-I strain, ST-7, Sm^r

[10]

Bbr79

401

Dog

Complex-I strain, ST-7, Sm^r

[10]

545

Pig

Complex-I strain, ST-7, Sm^r

[10]

548

Pig

Complex-I strain, ST-4, Sm^r

[10]

599

Dog

Complex-I strain, ST-27, Sm^r

[10]

601

Dog

Complex-I strain, ST-4, Sm^r

[10]

705

Rabbit

Complex-I strain, ST-10, Sm^r

[10]

723

Cat

Complex-I strain, ST-23, Sm^r

[10]

782

Cat

Complex-I strain, ST-5, Sm^r

[10]

BBE001

Human

Complex I, ST-11

[34]

BBF579

Human

Complex IV, Novel ST

[34]

Mammalian cells

HeLa/CCL-2^TM

Human cervical adenocarcinoma cell line

ATCC

A549/CCL-185^TM

Human lung carcinoma cell line

ATCC

J774A.1/ TIB-67^TM

Mouse monocyte-macrophage cell line

ATCC

Plasmids

pEGBR1005

pSS1129 based suicide plasmid harboring bscN in-frame deletion of codons 171–261, pir dependent, oriT, oriV, sacB, Km^R

[15]

pRE112-ΔbteA

pGP704 based suicide plasmid harboring bteA in-frame deletion of codons 4–653, pir dependent, oriT, oriV, sacB, Cm^R

[11]

pBBR1MCS-5

lacPOZ’ mob⁺, broad-host- cloning vector, Gm^R

[50]

pbteA

bteA cloned into pBBR1MCS-5, Gm^R

[11]