Storage conditions of intestinal microbiota matter in metagenomic analysis

BMC Microbiology

Table 3 Taxonomic comparison for 3 main bacterial taxa between frozen and RT samples

Taxon	F*	RT3h*	RT24h*	RT2w*	pvalue F vs RT3h	pvalue F vs RT24h	pvalue F vs RT2w
Bacteroides;uncultured bacterium	19	20	19	13	0.749	0.749	0.0349
Prevotellaceae;uncultured;human gut metagenome	7	6	5	3	0.6804	0.3189	0.0140
Bifidobacterium;uncultured bacterium	2	2	3	7	1	0.3964	0.0030

Statistical analysis was performed using Poisson regression model. * Values are mean proportion of sequences (%). p-value < 0.05 is considered significant; n = 4 subjects; F = frozen; UF1h = unfrozen during 1 h; UF3h = unfrozen during 3 h; RT = room temperature; 2w = 2 weeks; Taxonomy is indicated at the genus level and if not possible at the family level.

ISSN: 1471-2180