Figure 6
One-step growth curve of ZZ1 on A. baumannii AB09V. Phage ZZ1 was mixed with strain AB09V at an MOI of approximately 10 at 37°C (The initial ratio of phage concentration to bacterial concentration is 6.6 × 108 PFU/ml: 7.0 × 107 CFU/ml). Then, the total phage activity (including infected bacterial cells and free phages) was determined periodically. The decline in the concentration of total phages occurred as a result of the binding of multiple viral particles to one susceptible bacterial cell followed by a rapid increase, resulting in release of phages by lysis of the infected bacterial cells. The ZZ1 latent period was approximately 9 min, and the burst size averaged 200 PFU per infected cell.