Analysis of a Clostridium difficile PCR ribotype 078 100 kilobase island reveals the presence of a novel transposon, Tn6164

Table 2 Detection of specific regions of Tn6164 in PCR ribotype 078 strains

Strain	PCR 1-2^*	PCR1-3^§	PCR 4-5^#	PCR 6-7	PCR 8-9^†	PCR 12-2‡
56/69	-	+	+	-	-	+
26222	-	+	+	-	-	+
26114	-	+	+	-	-	+
26247	-	+	+	-	-	+
26235	-	+	+	-	-	+
ES1203	-	+	+	-	-	n.t.
6065935	-	+	+	-	n.t.	n.t.
7047337	-	+	+	-	n.t.	n.t.
8088158	-	+	+	-	n.t.	n.t.
50/19	-	+	+	+	+	-
GR0106	-	+	+	+	+	n.t.
DE1210	-	+	+	+	+	n.t.
BG1209	-	+	+	+	+	n.t.
NO1311	-	+	+	+	+	n.t.
NO1307	-	+	+	+	+	n.t.
IE1102	-	+	+	+	+	n.t.
GR0301	-	+	+	+	+	n.t.
10053737	-	+	+	+	n.t.	n.t.

^*PCR only positive when no insert is present, ^§PCR only positive when insert is present ^#PCR detects Module B, ^¶PCR detects module E, ^†PCR detects module D. ‡ PCR only positive in strains containing half of the element. Location of the oligonucleotides used is indicated in Figure 1. +, PCR positive; -, PCR negative; n.t., not tested.

ISSN: 1471-2180