Figure 5

Kinetic parameters of recombinant Pc Aad1p for Veratraldehyde and Veratryl alcohol. The kinetic parameters of the Pc Aad1 enzyme were determined for (A) the reduction reaction of Veratraldehyde and (B) the oxidation reaction of Veratryl alcohol. Activities were measured at 30°C in 50 mM MES buffer at pH 6.1 containing 0.3 mM NADPH in the reduction sense and in 100 mM Glycine-KOH buffer at pH 10.3 with 0.3 mM NADP⁺ for the oxidation reactions. The kinetic parameters for other substrates are presented in Table 2. Results are the mean ± SEM from at least three separate experiments.